PHYLOGENETIC DIVERSITY AND ANTIBIOTIC RESISTANCE OF UROPATHOGENIC ESCHERICHIA COLI AMONG PATIENTS IN DIYALA

Abstract

Background and Objectives: Escherichia coli considered most important cause of Urinary tract the assessment of antimicrobial susceptibility is essential for establishing the extent of the issue and choosing suitable antimicrobial agents. The current study aimed to map the spread of UroPathogenic E. coli (UPEC) based on genetic profiles and identify resistance patterns among the isolates. The study also wanted to find the phylogenetic groups of E. coli in Baquba, Iraq, using the Claremont phylogenetic classification method and look at how resistant they were to antibiotics.

Materials and Methods: This study employed biological correlations to study the patterns of antibiotic resistance and the distribution of phylogenetic groups of 105 isolates of E. coli and the relationship between them, which were isolated from two hospitals in Diyala, Iraq.

Results: The results of phylogenetic analysis of E. coli isolates Phylogenetic group B2 was the most prevalent (40%), B1 and D (each (20%), and C and A (6.6%). Ampicillin showed highest resistance against antibiotics, while imipenem the lowest resistance .were the percentage equal to 95% and 54.3 % respectively.  Group B2 showed the highest prevalence of antibiotic resistance, reaching 40%. Accordingly, 57 (54.3%) isolates were MDR and 40 (38.1%) were multidrug resistant. Furthermore, only 24 isolates (22.8%) were persistent and survived in stressful environments.

Conclusions. Our findings revealed a high prevalence of MDR E. coli isolates, with group B2 dominating. Such studies need to be done also in other regions to provide a greater understanding of the antibiotic resistance pattern and the prevalences of different phylogenetic groups